Anti-ALDH3A1 Antibody (CAB5502)

Antibody Name:	Anti-ALDH3A1 Antibody
Antibody SKU:	CAB5502
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ALDH3A1 (NP_001128640.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, NCI-H460, HT-29, HeLa, Mouse lung, Mouse testis, Mouse stomach, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ALDH3A1 (NP_001128640.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKI SEAV KRAR AAFS SGRT RPLQ FRIQ QLEA LQRL IQEQ EQEL VGAL AADL HKNE WNAY YEEV VYVL EEIE YMIQ KLPE WAAD EPVE KTPQ TQQD ELYI HSEP LGVV LVIG TWNY PFNL TIQP MVGA IAAG NSVV LKPS ELSE NMAS LLAT IIPQ YLDK DLYP VING GVPE TTEL LKER FDHI LYTG STGV GKII MTAA AKHL TPVT LELG GKSP CYVD KNCD LDVA CRRI AWGK FMNS GQTC VAPD YILC DPSI QNQI VEKL KKSL KEFY GEDA KKSR DYGR IISA RHFQ RVMG LIEG
Gene ID:	218
Uniprot:	P30838
Cellular Location:	Cytoplasm
Calculated MW:	50kDa
Observed MW:	55kDa

Synonyms:

ALDH3A1, ALDH3, ALDHIII

Background:

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	ALDH3A1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. Belongs to the aldehyde dehydrogenase family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - phenylalanine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.5; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; Oxidoreductase Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane Molecular Function:3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; benzaldehyde dehydrogenase (NAD+) activity; protein binding Biological Process: aldehyde metabolic process; xenobiotic metabolic process
NCBI Summary:	Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]
UniProt Code:	P30838
NCBI GenInfo Identifier:	311033473
NCBI Gene ID:	218
NCBI Accession:	P30838.3
UniProt Secondary Accession:	P30838,Q9BT37, A8K828,
UniProt Related Accession:	P30838
Molecular Weight:	50,395 Da
NCBI Full Name:	Aldehyde dehydrogenase, dimeric NADP-preferring
NCBI Synonym Full Names:	aldehyde dehydrogenase 3 family member A1
NCBI Official Symbol:	ALDH3A1
NCBI Official Synonym Symbols:	ALDH3; ALDHIII
NCBI Protein Information:	aldehyde dehydrogenase, dimeric NADP-preferring
UniProt Protein Name:	Aldehyde dehydrogenase, dimeric NADP-preferring
UniProt Synonym Protein Names:	ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1
Protein Family:	Aldehyde dehydrogenase
UniProt Gene Name:	ALDH3A1
UniProt Entry Name:	AL3A1_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH3A1 antibody (CAB5502) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using ALDH3A1 antibody (CAB5502). Blue: DAPI for nuclear staining.

Antibodies

Anti-ALDH3A1 Antibody (CAB13275)

ALDH3A1 Antibody (PACO01744)

ALDH3A1 Antibody (PACO07684)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)