Confocal imaging of Hep G2 cells using Alpha-1 Antitrypsin (SERPINA1) Rabbit mAb (CAB21972, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
The Alpha-1 Antitrypsin (SERPINA1) Monoclonal Antibody (CAB21972) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, exhibits high specificity and reactivity towards human samples, making it a reliable tool for Western blot and immunohistochemistry applications.Alpha-1 Antitrypsin, also known as AAT or SERPINA1, is an important serum protein that protects tissues from enzyme damage, particularly in the lungs and liver. Dysregulation of AAT has been implicated in various diseases such as emphysema, liver cirrhosis, and certain cancers, making it a valuable target for therapeutic interventions and diagnostic assays.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
5265
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells using Alpha-1 Antitrypsin (SERPINA1) Rabbit mAb (CAB21972) at 1:63000 dilution . Hep G2 treated with PNGase F (6 U/ul) at 37℃ for 1 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Western blot analysis of various lysates using Alpha-1 Antitrypsin (SERPINA1) Rabbit mAb (CAB21972) at 1:63000 dilution . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): RD Exposure time: 30 s.
