Anti-SERPINA1 Antibody (CAB12481)

Antibody Name:	Anti-SERPINA1 Antibody
Antibody SKU:	CAB12481
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-315 of human SERPINA1 (NP_000286.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HT-29, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-315 of human SERPINA1 (NP_000286.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EDPQ GDAA QKTD TSHH DQDH PTFN KITP NLAE FAFS LYRQ LAHQ SNST NIFF SPVS IATA FAML SLGT KADT HDEI LEGL NFNL TEIP EAQI HEGF QELL RTLN QPDS QLQL TTGN GLFL SEGL KLVD KFLE DVKK LYHS EAFT VNFG DTEE AKKQ INDY VEKG TQGK IVDL VKEL DRDT VFAL VNYI FFKG KWER PFEV KDTE EEDF HVDQ VTTV KVPM MKRL GMFN IQHC KKLS SWVL LMKY LGNA TAIF FLPD EGKL QHLE NELT HDII TKFL ENED RRSA SLHL PKL
Gene ID:	5265
Uniprot:	P01009
Cellular Location:	Endoplasmic reticulum, Secreted, extracellular matrix, extracellular space
Calculated MW:	34kDa/40kDa/46kDa
Observed MW:	45kDa, 50kDa

Synonyms:	SERPINA1, A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT
Background:	The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Ref.17 Ref.18 Ref.24Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). Ref.17 Ref.18 Ref.24
UniProt Protein Details:	Subcellular location: Secreted Ref.24. Short peptide from AAT: Secreted extracellular space extracellular matrix Ref.24. Tissue specificity: Plasma. Domain: The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. Post-translational modification: Several isomers are observed, resulting from the combination of different N-linked glycan structures and mature N-terminus. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary, whereas glycan at Asn-70 is di-antennary with trace amounts of tri-antennary, and glycan at Asn-271 is exclusively di-antennary. The structure of the antennas is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennas are fucosylated, which forms a Lewis-X determinant.Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. Polymorphism: The sequence shown is that of the M1V allele which is the most common form of PI (44 to 49%). Other frequent alleles are: M1A 20 to 23%; M2 10 to 11%; M3 14 to 19%. Involvement in Disease: Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD) [ MIM:613490]. A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Ref.58 Ref.60 Ref.62 Miscellaneous: The aberrant form is found in the plasma of chronic smokers, and persists after smoking is ceased. It can still be found ten years after smoking has ceased. Sequence similarities: Belongs to the serpin family. Sequence caution: The sequence CAD62334.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD62585.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
NCBI Summary:	The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
UniProt Code:	P01009
NCBI GenInfo Identifier:	1703025
NCBI Gene ID:	5265
NCBI Accession:	P01009.3
UniProt Secondary Accession:	P01009,Q0PVP5, Q13672, Q53XB8, Q5U0M1, Q7M4R2, Q86U18 Q86U19, Q96BF9, Q96ES1, A6PX14, B2RDQ8,
UniProt Related Accession:	P01009,Q13747,Q2L9S7,Q3I0J7,Q9P173
Molecular Weight:	46,737 Da
NCBI Full Name:	Alpha-1-antitrypsin
NCBI Synonym Full Names:	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol:	SERPINA1
NCBI Official Synonym Symbols:	PI; A1A; AAT; PI1; A1AT; MGC9222; PRO2275; MGC23330; alpha1AT
NCBI Protein Information:	alpha-1-antitrypsin; serpin A1; OTTHUMP00000197150; OTTHUMP00000197151; OTTHUMP00000197152; OTTHUMP00000197153; OTTHUMP00000197154; alpha-1-antiproteinase; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
UniProt Protein Name:	Alpha-1-antitrypsin
UniProt Synonym Protein Names:	Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
Protein Family:	Alpha-1-antitrypsin
UniProt Gene Name:	SERPINA1
UniProt Entry Name:	A1AT_HUMAN

	Western blot analysis of extracts of various cell lines, using SERPINA1 antibody (CAB12481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA1 antibody (CAB12481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody (CAB12481) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of PC-12 cells using SERPINA1 antibody (CAB12481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SERPINA1 Antibody (CAB1015)	Human Alpha-1-antitrypsin (SERPINA1) ELISA Kit
Anti-SERPINA1 Antibody (CAB4966)
SERPINA1 Antibody (PACO01775)
SERPINA1 Antibody (PACO02226)
SERPINA1 Antibody (PACO12105)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)