The 2'-O-Methylguanosine(Gm) Monoclonal Antibody (CAB20696) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting.2'-O-Methylguanosine is known to play important roles in RNA processing and function, making it a key molecule of interest in the fields of molecular biology and biochemistry. This antibody specifically binds to 2'-O-Methylguanosine, allowing for accurate detection and analysis of this modified nucleoside in various biological samples.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
2'-O-Methylguanosine(Gm) Monoclonal Antibody
SKU:
CAB20696
Size:
20μL, 100μL
Reactivity:
Species independent
Clone Number:
ARC50772
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to 2'-O-Methylguanosine(Gm).
Sequence:
Gm c onju gate d to KLH
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
Purification Method
Affinity purification
Gene ID
CAS: 2140-71-8
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
The 2'-O-methylguanosine(Gm) Rabbit mAb (CAB20696) are tested in Dot Blot against 2'-O-methylguanosine and unmodified guanosine.2'-O-methylguanosine：Biotin-5'GCATAATGACTACT(Gm)3'unmodified guanosine：Biotin-5'GCATAATGACTACTG3'
