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Acetyl-Histone H4-K5/K8/K12/K16 Polyclonal Antibody (CAB20764)

SKU:
CAB20764
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Alkaline Phosphatase conjugated IgG
€179 - €469
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Acetyl-Histone H4-K5/K8/K12/K16 Polyclonal Antibody
SKU:CAB20764
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic acetylated peptide around K5/K8/K12/K16 of human Histone H4.
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; Acetyl-Histone H4-K5/K8/K12/K16
Positive Sample:NIH/3T3 treated by TSA,C6 treated by TSA
Conjugate:Unconjugated
Cellular Localization:extracellular exosome, extracellular region, nucleoplasm, nucleus
Calculated MW:11kDa
Observed MW:Refer to figures

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Purification Method:Affinity purification
Gene ID:8359
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb (CAB20764) at 1:500 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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