The FACL4 Monoclonal Antibody (CAB20414) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ACSL4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in fields such as biochemistry and cell biology.ACSL4, also known as long-chain acyl-CoA synthetase 4, plays a critical role in lipid metabolism by catalyzing the formation of fatty acyl-CoA from long-chain fatty acids.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FACL4 Monoclonal Antibody
SKU:
CAB20414
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC53209
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACS4, FACL4, LACS4, MRX63, MRX68, XLID63, ACSL4
Positive Sample:
Hep G2, Mouse liver, Rat liver, Mouse kidney
Cellular Localization:
Endoplasmic Reticulum Membrane, Microsome Membrane, Mitochondrion Outer Membrane, Peroxisome Membrane, Single-Pass Type Iii Membrane Protein.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2182
RRID
AB_2909505
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACSL4 Rabbit mAb (CAB20414) at 1:58000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from Rat liver using ACSL4 Rabbit mAb (CAB20414) at 1:58000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Confocal imaging of Hep G2 cells using ACSL4 Rabbit mAb (CAB20414, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation analysis of 300 μg extracts of HepG2 cells using 3 μg ACSL4 Rabbit mAb (CAB20414). Western blot was performed from the immunoprecipitate using ACSL4 Rabbit mAb (CAB20414) at a dilution of 1:1000.
