The ACSL4 Polyclonal Antibody (CAB20414) is a valuable tool for researchers studying ACSL4, an enzyme involved in lipid metabolism and inflammation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ACSL4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in fields such as biochemistry and cell biology.ACSL4, also known as long-chain acyl-CoA synthetase 4, plays a critical role in lipid metabolism by catalyzing the formation of fatty acyl-CoA from long-chain fatty acids.
Dysregulation of ACSL4 has been implicated in various diseases, including cancer and metabolic disorders. Research into the function and regulation of ACSL4 is vital for understanding its role in these conditions and developing potential therapeutic strategies.By utilizing the ACSL4 Polyclonal Antibody, researchers can gain valuable insights into the function of ACSL4 in normal physiological processes and disease states. This antibody offers a powerful tool for investigating the role of ACSL4 in lipid metabolism and inflammation, paving the way for advancements in drug discovery and personalized medicine.
Product Name:
FACL4 Rabbit Monoclonal Antibody
SKU:
CAB20414
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1).
WB,1:2000 - 1:5000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ACS4; FACL4; LACS4; MRX63; MRX68; XLID63; ACSL4
Positive Sample:
Hep G2,Mouse liver,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2182
Clone Number:
ARC53209
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ACSL4 Rabbit mAb (CAB20414) at 1:4600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.3s.
