The CASTOR1 Polyclonal Antibody (CAB20710) is a high-quality antibody developed for reliable detection and analysis of target proteins. Castor1 is a key regulator of autophagy, a cellular process crucial for maintaining cellular homeostasis and dealing with stress conditions. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.The Castor1 protein plays a critical role in the autophagy pathway, helping to regulate the process of self-degradation in response to various stimuli such as nutrient deprivation or infection.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CASTOR1 Polyclonal Antibody
SKU:
CAB20710
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GATSL3, CASTOR1
Positive Sample:
Jurkat, Mouse lung
Cellular Localization:
Cytosol.
Calculated MW:
36kDa
Observed MW:
39kDa
Enables arginine binding activity and identical protein binding activity. Involved in cellular response to L-arginine and negative regulation of TORC1 signaling. Located in cytosol. Colocalizes with GATOR2 complex.
Purification Method
Affinity purification
Gene ID
652968
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CASTOR1 Rabbit pAb (CAB20710) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
