The CTNNA3 Monoclonal Antibody (CAB19282) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been shown to be highly reactive with human samples, making it a reliable tool for Western blot applications.CTNNA3 is an important component in cell adhesion complexes, contributing to the structural integrity of tissues and regulating cell communication. Its involvement in cell signaling pathways suggests a potential role in various cellular processes, making it a valuable target for research in developmental biology, cancer biology, and tissue engineering.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CTNNA3 Monoclonal Antibody
SKU:
CAB19282
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2463
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VR22, ARVD13, CTNNA3
Positive Sample:
PC-3, Mouse testis, Rat testis, Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
100kDa
Observed MW:
100kDa
This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
29119
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain, using CTNNA3 Rabbit mAb (CAB19282) at 1 : 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates, using CTNNA3 Rabbit mAb (CAB19282) at 1 : 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using CTNNA3 Rabbit mAb (CAB19282) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CTNNA3 Rabbit mAb (CAB19282) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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