The CTNNA3 Monoclonal Antibody (CAB19282) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants. RRID Gene ID 29119 Swiss Prot Synonym VR22; ARVD13; CTNNA3
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CTNNA3 Monoclonal Antibody
SKU:
CAB19282
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2463
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VR22, ARVD13, CTNNA3
Positive Sample:
PC-3, Mouse testis, Rat testis, Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
100kDa
Observed MW:
100kDa
This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants. RRID Gene ID 29119 Swiss Prot Synonym VR22; ARVD13; CTNNA3
Purification Method:
Affinity purification
Gene ID:
29119
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain, using CTNNA3 Rabbit mAb (CAB19282) at 1 : 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates, using CTNNA3 Rabbit mAb (CAB19282) at 1 : 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using CTNNA3 Rabbit mAb (CAB19282) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CTNNA3 Rabbit mAb (CAB19282) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
