The HGD Monoclonal Antibody (CAB1400) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting. By binding to the HGD protein, this antibody enables detection and analysis in different cell types, making it suitable for studies in metabolic disorders and related diseases. HGD, also known as homogentisate 1,2-dioxygenase, plays a crucial role in the breakdown of tyrosine and is essential for the proper functioning of the tyrosine degradation pathway.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HGD Monoclonal Antibody
SKU:
CAB1400
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2561
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AKU, HGO, HGD
Positive Sample:
HT-29, HepG2
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.
Purification Method
Affinity purification
Gene ID
3081
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HGD Rabbit mAb (CAB1400) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
