The Histone H4 Monoclonal Antibody (CAB19815) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with samples from various species and has been validated for use in Western blot and immunofluorescence applications.Histone H4 is a key component of nucleosomes, which are the building blocks of chromatin and play a fundamental role in DNA packaging. Aberrant histone H4 function has been linked to various diseases, including cancer, making it a relevant target for investigation in cancer research and epigenetics studies.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Histone H4 Monoclonal Antibody
SKU:
CAB19815
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:
ARC2340
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Purification Method
Affinity purification
Gene ID
8359
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Histone H4 Rabbit mAb (CAB19815) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Histone H4 Rabbit mAb (CAB19815) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H4 Rabbit mAb (CAB19815) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
