null

Anti-HK1 Antibody (CAB20796)

SKU:
CAB20796
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Alexa Fluor 488 conjugated IgG
  • Immunofluorescence analysis of A549 using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U-2 OS using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
€179 - €469
Frequently bought together:

Description

Antibody Name:Anti-HK1 Antibody (CAB20796)
Antibody SKU:CAB20796
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, U-87MG, 293T, Mouse brain, Mouse heart, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KIDK YLYA MRLS DETL IDIM TRFR KEMK NGLS RDFN PTAT VKML PTFV RSIP DGSE KGDF IALD LGGS SFRI LRVQ VNHE KNQN VHME SEVY DTPE NIVH GSGS QLFD HVAE CLGD FMEK RKIK DKKL PVGF TFSF PCQQ SKID EAIL ITWT KRFK ASGV EGAD VVKL LNKA IKKR GDYD ANIV AVVN DTVG TMMT CGY
Cellular Location:Mitochondrion outer membrane
Calculated MW:101kDa/102kDa
Observed MW:102kDa
Synonyms:HK1, HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase, RP79
Background:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Immunofluorescence analysis of A549 using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of A549 using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of U-2 OS using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U-2 OS using HK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.Western blot analysis of extracts of various cell lines, using HK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Related Products