Anti-HK1 Antibody Antibody

Antibody Name:	Anti-HK1[KO Validated] Antibody
Antibody SKU:	CAB0533
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Hexokinase 1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7, Mouse testis, Mouse brain, Rat testis, Rat brain

Immunogen:	A synthesized peptide derived from human Hexokinase 1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3098
Uniprot:	P19367
Cellular Location:
Calculated MW:	102kDa
Observed MW:	120KDa

	Western blot analysis of extracts from normal (control) and HK1 knockout (KO) 293T cells, using HK1 Rabbit mAb (CAB0533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using HK1 Rabbit mAb (CAB0533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using HK1 Rabbit mAb (CAB0533) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using HK1 Rabbit mAb (CAB0533) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HK1 Rabbit mAb (CAB0533) at dilution of 1:100 (40x lens).

Synonyms:

HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase

Background:

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

NCBI Summary:	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
UniProt Code:	P19367
NCBI GenInfo Identifier:	116242516
NCBI Gene ID:	3098
NCBI Accession:	P19367.3
UniProt Secondary Accession:	P19367,O43443, O43444, O75574, Q5VTC3, Q96HC8, Q9NNZ4 Q9NNZ5, E9PCK0,
UniProt Related Accession:	P19367
Molecular Weight:	101,085 Da
NCBI Full Name:	Hexokinase-1
NCBI Synonym Full Names:	hexokinase 1
NCBI Official Symbol:	HK1
NCBI Official Synonym Symbols:	HK; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
NCBI Protein Information:	hexokinase-1
UniProt Protein Name:	Hexokinase-1
UniProt Synonym Protein Names:	Brain form hexokinase; Hexokinase type I; HK I
Protein Family:	Probable histidine kinase
UniProt Gene Name:	HK1
UniProt Entry Name:	HXK1_HUMAN

Antibodies	ELISA Kits
Anti-HK1 Antibody (CAB1054)[KO Validated]	Human HK1 (Hexokinase-1) ELISA Kit
HK1 Antibody (PACO06571)	Human Hexokinase-1 (HK1) ELISA Kit
HK1 Antibody (PACO09734)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)