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[KO Validated] HK1 Rabbit Monoclonal Antibody (CAB0533)

SKU:
CAB0533
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Metabolism
  • KO Validated Antibodies 1 Anti-HK1KO Validated Antibody CAB0533
  • KO Validated Antibodies 1 Anti-HK1KO Validated Antibody CAB0533
  • KO Validated Antibodies 1 Anti-HK1KO Validated Antibody CAB0533
  • KO Validated Antibodies 1 Anti-HK1KO Validated Antibody CAB0533
  • KO Validated Antibodies 1 Anti-HK1KO Validated Antibody CAB0533
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] HK1 Rabbit Monoclonal Antibody
SKU:CAB0533
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367).
Sequence:GLSR DFNP TATV KMLP TFVR SIPD GSEK GDFI ALDL GGSS FRIL RVQV NHEK NQNV HMES EVYD TPEN IVHG SGSQ LFDH VAEC LGDF MEKR KIKD KKLP V
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:HK; HKD; HKI; HXK1; NMSR; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase; K1
Positive Sample:293T,MCF7,Mouse testis,Mouse brain,Rat testis,Rat brain
Conjugate:Unconjugated
Cellular Localization:Mitochondrion outer membrane.
Calculated MW:102kDa
Observed MW:120kDa

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Purification Method:Affinity purification
Gene ID:3098
Clone Number:ARC0256
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit mAb (CAB0533) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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