The HLA-DQB1 Antibody (CAB20372) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in a variety of research applications, including Western blot analysis.HLA-DQB1 plays a crucial role in immune recognition and response, particularly in the context of antigen presentation and T cell activation. By targeting this molecule with the Anti-HLA-DQB1 Antibody, researchers can gain valuable insights into the mechanisms underlying immune function and dysfunction.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HLA-DQB1 Antibody
SKU:
CAB20372
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IDDM1, CELIAC1, HLA-DQB, HLA-DQB1
Positive Sample:
Raji
Cellular Localization:
Plasma Membrane.
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3119
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using HLA-DQB1 Rabbit pAb (CAB20372) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using HLA-DQB1 Rabbit pAb (CAB20372) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
