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LEO1 Rabbit Polyclonal Antibody (CAB8806)

SKU:
CAB8806
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
LEO1
RDL
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:LEO1 Rabbit Polyclonal Antibody
SKU:CAB8806
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 340-570 of human LEO1 (NP_620147.1).
Sequence:ENGL PQDQ QEEE PIPE TRIE VEIP KVNT DLGN DLYF VKLP NFLS VEPR PFDP QYYE DEFE DEEM LDEE GRTR LKLK VENT IRWR IRRD EEGN EIKE SNAR IVKW SDGS MSLH LGNE VFDV YKAP LQGD HNHL FIRQ GTGL QGQA VFKT KLTF RPHS TDSA THRK MTLS LADR CSKT QKIR ILPM AGRD PECQ RTEM IKKE EERL RASI RRES QQRR MREK QHQR GLSA SYL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:RDL; LEO1
Positive Sample:HepG2,Jurkat,Mouse liver,Mouse brain,Rat brain
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:75kDa
Observed MW:105kDa

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).

Purification Method:Affinity purification
Gene ID:123169
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using LEO1 Rabbit pAb (CAB8806) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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