The MDH1 Monoclonal Antibody (CAB9673) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the MDH1 protein, it allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in biochemistry and cancer research.MDH1 is essential for converting malate to oxaloacetate, a crucial step in cellular energy production. Dysregulation of MDH1 has been implicated in various diseases, including cancer and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MDH1 Monoclonal Antibody
SKU:
CAB9673
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1692
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HeLa, Jurkat, U-251MG, Mouse liver, Mouse brain, Rat kidney, Rat heart
Cellular Localization:
Cytoplasm.
Calculated MW:
36kDa
Observed MW:
36kDa
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Purification Method
Affinity purification
Gene ID
4190
RRID
AB_2863757
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MDH1 Rabbit mAb (CAB9673) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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