Description
Anti-Mouse CD370 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD370 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody ideal for in vivo research applications. This antibody targets CD370 (also known as CLEC9A), a critical protein involved in the immune system's response, making it highly suitable for studies in immunology and related disciplines. With a mouse IgG2a isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for various assays, including ELISA, flow cytometry, and immunohistochemistry. Available in multiple sizes, it is formulated in phosphate-buffered saline to guarantee stability and efficacy.
Elevate your research with the reliability and flexibility of this versatile antibody. CD370 is predominantly expressed on dendritic cells and plays a pivotal role in the immune response by recognizing dead and damaged cells, thus facilitating their clearance and subsequent immune activation. This function underscores its importance in both innate and adaptive immunity, making the Anti-Mouse CD370 antibody a valuable tool for advanced immunological research. ---
| Product Name: | Anti-Mouse CD370 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0169 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 1F6 |
| Protein: | CD370 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD370, DNGR-1, C-Type Lectin Domain Family 9 Member A |
| Isotype: | Rat IgG1 |
| Reactivity: | Mouse |
| Immunogen: | RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope |
| Applications: | FC, IF, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, IF, WB |
| Recommended Usage: | FC WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | This clone 1F6 monoclonal antibody recognizes a direct epitope on mouse DNGR1/CLEC9A for CD8+ dendritic cells, which can be exploited for tumor therapy, whilst also recognizing necrotic cells. This antibody can recognize both the short and long isoform of DNGR-1, which varies in a segment of the stalk region. |
| Antigen Distribution: | CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs |
| Immunogen: | RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CLEC9A antibody, 1F6, recognizes C-type lectin domain family 9 member A (CLEC9A), also known as CD370 and DNGR-1. CLEC9A is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. In mice and humans, CLEC9A is expressed by CD8a+ conventional dendritic cells (cDCs) in mice1,2 and BDCA3+ DCs in humans3, which are specialized for the cross-presentation of exogenous antigens on MHC class I4. In mice, type I interferon (IFN)-secreting plasmacytoid DCs (pDCs) also express CLEC9A2. The ligand for CLEC9A is filamentous actin exposed on apoptotic cells5,6, and ligation of CLEC9A results in the cross-presentation and induction of CD8 T cell responses. CLEC9A is a target for vaccine enhancement, and antibodies targeting CLEC9A in vaccination studies lead to enhanced presentation by DCs, eliciting CD8 and CD4 T cell proliferation and strong humoral immunity. In addition, antigen targeting DCs via CLEC9A can enhance anti-tumor immunity12.
| Datasheet: | View |
| Protein: | CD370 |
| Research Area: | Immunology, Innate Immunity |
