The NKX6-1 Antibody (CAB20419) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for human samples, making it ideal for use in experiments such as Western blotting.NKX6-1 is known for its role in regulating insulin production and glucose metabolism, making it a key player in understanding diseases like diabetes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NKX6-1 Antibody
SKU:
CAB20419
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NKX6A, NKX6.1, NKX6-1
Positive Sample:
BxPC-3, Rat pancreas, 293T
Cellular Localization:
Extracellular Exosome, Nucleus.
Calculated MW:
38kDa
Observed MW:
45kDa/46kDa/45-52kDa
In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].
Purification Method
Affinity purification
Gene ID
4825
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells using NKX6-1 Rabbit pAb (CAB20419) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using NKX6-1 Rabbit pAb (CAB20419) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
