The PBRM1 Monoclonal Antibody (CAB0334) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot and immunohistochemistry applications.PBRM1, also known as BAF180, is a subunit of the SWI/SNF chromatin remodeling complex, and alterations in its function have been linked to various cancers, including renal cell carcinoma. The Anti-PBRM1 Antibody allows for the detection and analysis of PBRM1 protein levels in different cell types, making it an essential reagent for studies in cancer biology and epigenetics.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PBRM1 Monoclonal Antibody
SKU:
CAB0334
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1820
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PB1, RCC, BAF180, SMARCH1, PBRM1
Positive Sample:
Rat brain, HeLa, NIH/3T3
Cellular Localization:
Nucleus.
Calculated MW:
193kDa
Observed MW:
200kDa
This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma.
Purification Method
Affinity purification
Gene ID
55193
RRID
AB_2861458
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PBAbGn1 Rabbit mAb (CAB0334) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon using PBAbGn1 Rabbit mAb (CAB0334) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PBAbGn1 Rabbit mAb (CAB0334) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
