The Phospho-EGFR-Y1173 Antibody (CABP1213) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific for the phosphorylated tyrosine 1173 residue on the EGFR protein. It has been validated for use in Western blot applications and is particularly reactive with human samples.EGFR is a key player in various cellular processes, including cell growth, proliferation, and survival. Phosphorylation of specific tyrosine residues, such as Y1173, is crucial for activating downstream signaling cascades that regulate these functions. The Anti-Phospho EGFR (Y1173) Antibody allows for the detection and analysis of EGFR phosphorylation levels in different cell types, providing valuable insights into EGFR-mediated signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-EGFR-Y1173 Antibody
SKU:
CABP1213
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method
Affinity purification
Gene ID
1956
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1173 Rabbit pAb (CABP1213) at 1:500 dilution. A-431 cells were treated with EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
