Anti-SIRT3 Antibody [KO Validated] (CAB20805)

Antibody Name:	Anti-SIRT3 Antibody [KO Validated] (CAB20805)
Antibody SKU:	CAB20805
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunoprecipitation
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1).

Application:	Western blotting, Immunohistochemistry, Immunoprecipitation
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, HepG2, Mouse liver, Rat liver, Mouse kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QRFL LHVV DFPM ADLL LILG TSLE VEPF ASLT EAVR SSVP RLLI NRDL VGPL AWHP RSRD VAQL GDVV HGVE SLVE LLGW TEEM RDLV QRET GKLD GPDK
Cellular Location:	Mitochondrion matrix
Calculated MW:	28kDa/43kDa
Observed MW:	28kDa

Synonyms:

SIRT3, SIR2L3, sirtuin 3

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

	Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug [KO Validated] SIRT3 antibody . Western blot was performed from the immunoprecipitate using [KO Validated] SIRT3 antibody at a dilition of 1:1000.
	Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] SIRT3 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of Mouse kidney, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.