Anti-SIRT3 Antibody (CAB5718)

Antibody Name:	Anti-SIRT3 Antibody [KO Validated]
Antibody SKU:	CAB5718
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:20 - 1:50 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, Mouse skeletal muscle, Mouse kidney, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QRFL LHVV DFPM ADLL LILG TSLE VEPF ASLT EAVR SSVP RLLI NRDL VGPL AWHP RSRD VAQL GDVV HGVE SLVE LLGW TEEM RDLV QRET GKLD GPDK
Gene ID:	23410
Uniprot:	Q9NTG7
Cellular Location:	Mitochondrion matrix
Calculated MW:	28kDa/43kDa
Observed MW:	28KDa/44KDa

Synonyms:

SIRT3, SIR2L3, sirtuin 3

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

UniProt Code:	Q9NTG7
NCBI GenInfo Identifier:	38258651
NCBI Gene ID:	23410
NCBI Accession:	Q9NTG7.2
UniProt Secondary Accession:	Q9NTG7,Q9Y6E8, B7Z5U6,
UniProt Related Accession:	Q9NTG7
Molecular Weight:	399
NCBI Full Name:	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
NCBI Synonym Full Names:	sirtuin 3
NCBI Official Symbol:	SIRT3
NCBI Official Synonym Symbols:	SIR2L3
NCBI Protein Information:	NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
UniProt Protein Name:	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
UniProt Synonym Protein Names:	Regulatory protein SIR2 homolog 3; SIR2-like protein 3
Protein Family:	NAD-dependent protein deacetylase sirtuin
UniProt Gene Name:	SIRT3
UniProt Entry Name:	SIR3_HUMAN

	Western blot analysis of extracts of 293T cells, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (CAB5718) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using SIRT3 antibody (CAB5718) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SIRT3 Antibody (CAB5419)	Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit
Anti-SIRT3 Antibody (CAB7307)
Anti-SIRT3 Antibody (CAB17113)
SIRT3 Antibody (PACO03698)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)