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Anti-UFD1L Antibody (CAB20977)

SKU:
CAB20977
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€179 - €469
Frequently bought together:

Description

Antibody Name:Anti-UFD1L Antibody (CAB20977)
Antibody SKU:CAB20977
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human UFD1L.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, K-562, Mouse brain, Rat testis
Immunogen:Recombinant protein of Human UFD1L.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Nucleus, cytosol
Calculated MW:29kDa/34kDa/38kDa
Observed MW:40KDa
Synonyms:UFD1, UFD1L
Background:The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
Western blot analysis of extracts of various cell lines, using UFD1L antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using UFD1L antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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