Anti-UFD1L Antibody (CAB3255)

Antibody Name:	Anti-UFD1L Antibody
Antibody SKU:	CAB3255
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, A-431, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFSF NMFD HPIP RVFQ NRFS TQYR CFSV SMLA GPND RSDV EKGG KIIM PPSA LDQL SRLN ITYP MLFK LTNK NSDR MTHC GVLE FVAD EGIC YLPH WMMQ NLLL EEGG LVQV ESVN LQVA TYSK FQPQ SPDF LDIT NPKA VLEN ALRN FACL TTGD VIAI NYNE KIYE LRVM ETKP DKAV SIIE CDMN VDFD APLG YKEP ERQV QHEE STEG EADH SGYA GELG FRAF SGSG NRLD GKKK GVEP SPSP IKPG DIKR GIPN YEFK LGKI TFIR NSRP LVKK VEED EAGG RFVA FSGE GQSL RKKG RKP
Gene ID:	7353
Uniprot:	Q92890
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	29kDa/34kDa/38kDa
Observed MW:	37kDa

Synonyms:

UFD1, UFD1L

Background:

The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.

UniProt Protein Function:	UFD1L: Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. Belongs to the UFD1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin-specific protease activity; protein complex binding; receptor binding Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; skeletal development
NCBI Summary:	The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Jun 2009]
UniProt Code:	Q92890
NCBI GenInfo Identifier:	160332310
NCBI Gene ID:	7353
NCBI Accession:	Q92890.3
UniProt Secondary Accession:	Q92890,Q9Y5N0, A8MW31,
UniProt Related Accession:	Q92890
Molecular Weight:	307
NCBI Full Name:	Ubiquitin fusion degradation protein 1 homolog
NCBI Synonym Full Names:	ubiquitin fusion degradation 1 like (yeast)
NCBI Official Symbol:	UFD1L
NCBI Official Synonym Symbols:	UFD1
NCBI Protein Information:	ubiquitin fusion degradation protein 1 homolog; UB fusion protein 1
UniProt Protein Name:	Ubiquitin fusion degradation protein 1 homolog
UniProt Gene Name:	UFD1L
UniProt Entry Name:	UFD1_HUMAN

	Western blot analysis of extracts of various cell lines, using UFD1L antibody (CAB3255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using UFD1L antibody (CAB3255) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using UFD1L antibody (CAB3255).

Antibodies

Anti-UFD1L Antibody (CAB6783)

UFD1L Antibody (PACO13073)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)