Western blot analysis of extracts from normal (control) and XIAP knockout (KO) 293T cells, using XIAP antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
The [KO Validated] XIAP Monoclonal Antibody (CAB20846) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in knockout-validated rabbits, exhibits high specificity for XIAP in human samples and is validated for use in Western blot applications.XIAP, also known as X-linked inhibitor of apoptosis protein, is a key player in the apoptotic process, blocking the activation of caspases and preventing cell death. Dysregulation of XIAP has been implicated in various diseases, including cancer, making it an attractive target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] XIAP Monoclonal Antibody
SKU:
CAB20846
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC51953
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
API3, ILP1, MIHA, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3, AP
Positive Sample:
HeLa, RAW 264.7, PC-12
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
53kDa
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
Purification Method
Affinity purification
Gene ID
331
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using [KO Validated] XIAP Rabbit mAb (CAB20846) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) HeLa cells, using [KO Validated] XIAP Rabbit mAb (CAB20846) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
