Western blot analysis of various lysates, using AP2B1 Rabbit mAb (CAB23784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The AP2B1 Monoclonal Antibody (CAB23784) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through hybridoma technology, is ideal for studying the role of AP2B1 in clathrin-mediated endocytosis, a fundamental process for the internalization of cell surface receptors and other molecules.AP2B1 is essential for the formation of clathrin-coated vesicles, which play a crucial role in various cellular processes such as receptor trafficking, nutrient uptake, and signal transduction. The AP2B1 Monoclonal Antibody is validated for use in immunofluorescence, immunoprecipitation, and other biochemical assays, enabling researchers to explore the function of AP2B1 in different cell types and experimental settings.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AP2B1 Monoclonal Antibody
SKU:
CAB23784
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC61904
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
163
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using AP2B1 Rabbit mAb (CAB23784) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
