Anti-AP2B1 Antibody (CAB1995)

Antibody Name:	Anti-AP2B1 Antibody
Antibody SKU:	CAB1995
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	22Rv1, A-549, K-562, HeLa, 293T, BT-474, U-251MG, Mouse brain, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEMN FTNK ALQH MTDF AIQF NKNS FGVI PSTP LAIH TPLM PNQS IDVS LPLN TLGP VMKM EPLN NLQV AVKN NIDV FYFS CLIP LNVL FVED GKME RQVF LATW KDIP NENE LQFQ IKEC HLNA DTVS SKLQ NNNV YTIA KRNV EGQD MLYQ SLKL TNGI WILA ELRI QPGN PNYT LSLK CRAP EVSQ YIYQ VYDS ILKN
Gene ID:	163
Uniprot:	P63010
Cellular Location:	Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Calculated MW:	98kDa/104kDa/105kDa
Observed MW:	105kDa

Synonyms:	AP2B1, ADTB2, AP105B, AP2-BETA, CLAPB1
Background:	The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	AP2B1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin- associated sorting proteins (CLASPs) are recognized by their [DE]- X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 17q12 Cellular Component: intracellular membrane-bound organelle; membrane; plasma membrane; AP-2 adaptor complex; cytosol Molecular Function:clathrin binding; protein binding; signal sequence binding; protein transporter activity Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; synaptic transmission; intracellular protein transport; axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus
NCBI Summary:	The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P63010
NCBI GenInfo Identifier:	51702211
NCBI Gene ID:	163
NCBI Accession:	P63010.1
UniProt Secondary Accession:	P63010,P21851, Q7Z451, Q96J19, A6NJP3,
UniProt Related Accession:	P63010
Molecular Weight:	937
NCBI Full Name:	AP-2 complex subunit beta
NCBI Synonym Full Names:	adaptor-related protein complex 2, beta 1 subunit
NCBI Official Symbol:	AP2B1
NCBI Official Synonym Symbols:	ADTB2; AP105B; CLAPB1; AP2-BETA
NCBI Protein Information:	AP-2 complex subunit beta; beta-adaptin; beta-2-adaptin; adaptin, beta 2 (beta); adaptor protein complex AP-2 subunit beta; adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptor-related protein complex 2 s
UniProt Protein Name:	AP-2 complex subunit beta
UniProt Synonym Protein Names:	AP105B; Adaptor protein complex AP-2 subunit beta; Adaptor-related protein complex 2 subunit beta; Beta-2-adaptin; Beta-adaptin; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor HA2/AP2 adaptin beta subunit
Protein Family:	AP-2 complex
UniProt Gene Name:	AP2B1
UniProt Entry Name:	AP2B1_HUMAN

	Western blot analysis of extracts of various cell lines, using AP2B1 antibody (CAB1995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (CAB1995) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (CAB1995) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (CAB1995) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
AP2B1 Antibody (PACO07767)	Human AP2B1 / AP-2 complex subunit beta ELISA Kit
	Human AP-2 complex subunit beta (AP2B1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)