Anti-ARAP1 Antibody (CAB10466)

SKU:
CAB10466
€99 - €249

Description

Technical Manual

Anti-ARAP1 Antibody

Product Name:Anti-ARAP1 Antibody
Product SKU:CAB10466
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3, 4, 5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).
Sequence:

SVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

Gene ID:116985
Swiss Prot:Q96P48
Synonyms:ARAP1; CENTD2
Calculated MW:77kDa/127kDa/135kDa/136kDa/160kDa/162kDa
Observed MW:190kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:293T, LO2, HeLa, Mouse liver, Rat liver
Cellular Location:Cell membrane, Cytoplasm, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein

Product Images

Anti-ARAP1 Antibody (CAB10466)

Western blot analysis of extracts of various cell lines, using ARAP1 antibody (CAB10466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Uniprot Information

UniProt Protein Function:CENTD2: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:GAP; GAP, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function:GTPase activator activity; phosphatidylinositol-3,4,5-triphosphate binding; protein binding

Biological Process: actin filament reorganization during cell cycle; negative regulation of stress fiber formation; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of filopodium formation; positive regulation of GTPase activity; regulation of cell motility; regulation of cell shape; regulation of small GTPase mediated signal transduction

NCBI Summary:The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:Q96P48
NCBI GenInfo Identifier:226694321
NCBI Gene ID:116985
NCBI Accession:Q96P48.3
UniProt Secondary Accession:Q96P48,O94879, Q4LDD5, Q59FI7, Q6PHS3, Q8WU51, Q96HP6 Q96L71, A3KLL7, B2RTS2,
UniProt Related Accession:Q96P48
Molecular Weight:162kDa
NCBI Full Name:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
NCBI Synonym Full Names:ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
NCBI Official Symbol:ARAP1
NCBI Official Synonym Symbols:CENTD2
NCBI Protein Information:arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
UniProt Protein Name:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
UniProt Synonym Protein Names:Centaurin-delta-2; Cnt-d2
Protein Family:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
UniProt Gene Name:ARAP1
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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