Anti-ARF6 Antibody (CAB3162)

Antibody Name:	Anti-ARF6 Antibody
Antibody SKU:	CAB3162
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ARF6

Application:	WB IHC
Recommended Dilution:	WB 1:100 - 1:500 IHC 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	A-549, MCF7, U-251MG, HepG2, HeLa

Immunogen:	A synthetic peptide of human ARF6
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	382
Uniprot:	P62330
Cellular Location:	Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane
Calculated MW:	20kDa
Observed MW:	20kDa

Synonyms:

ARF6

Background:

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

UniProt Protein Function:	GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1.
NCBI Summary:	This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
UniProt Code:	P62330
NCBI GenInfo Identifier:	51316984
NCBI Gene ID:	382
NCBI Accession:	P62330.2
UniProt Secondary Accession:	P62330,P26438, Q6FGZ2,
UniProt Related Accession:	P62330
Molecular Weight:	20,082 Da
NCBI Full Name:	ADP-ribosylation factor 6
NCBI Synonym Full Names:	ADP ribosylation factor 6
NCBI Official Symbol:	ARF6
NCBI Protein Information:	ADP-ribosylation factor 6
UniProt Protein Name:	ADP-ribosylation factor 6
Protein Family:	Auxin response factor
UniProt Gene Name:	ARF6

	Western blot analysis of extracts of various cell lines, using ARF6 antibody (CAB3162) at 1:250 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using ARF6 Antibody (CAB3162) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using ARF6 Antibody (CAB3162) at dilution of 1:100 (40x lens).

Antibodies

Anti-ARF6 Antibody (CAB0988)

Anti-ARF6 Antibody (CAB11485)

ARF6 Antibody (PACO04176)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)