Description
ATF2 Transcription Factor Activity Assay
The ATF2 (Transcription Factor Activity) Assay Kit is a reliable and efficient tool for measuring ATF2 transcription factor activity in biological samples. This kit is specifically designed for researchers looking to study the role of ATF2 in gene regulation, cell growth, and cellular stress responses.ATF2 is a key transcription factor that plays a critical role in controlling the expression of genes involved in cell survival, apoptosis, and DNA damage response. Dysregulation of ATF2 activity has been linked to various diseases, including cancer and neurodegenerative disorders, making it a valuable target for therapeutic interventions.With its high sensitivity and specificity, the ATF2 Assay Kit delivers accurate and reproducible results, enabling researchers to elucidate the molecular mechanisms underlying ATF2 signaling pathways.
This kit is suitable for use with a variety of sample types, including cell lysates, tissue homogenates, and purified protein samples, making it a versatile tool for studying ATF2 function in different biological contexts.Overall, the ATF2 Assay Kit is a valuable resource for researchers seeking to gain insights into the role of ATF2 in cellular processes and disease pathogenesis. Its user-friendly format and reliable performance make it an essential tool for advancing our understanding of ATF2 biology and developing novel therapeutic strategies.
| Product Name: | ATF2 Transcription Factor Activity Assay |
| Product Code: | TFAB00083 |
| Target: | ATF2 |
| Synonyms: | Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ATF2 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 2q32 Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; cAMP response element binding protein binding; transcription coactivator activity; chromatin binding; transcription factor activity; protein kinase binding Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of transforming growth factor-beta2 production; response to osmotic stress; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; histone acetylation; toll-like receptor 9 signaling pathway; response to DNA damage stimulus; toll-like receptor 4 signaling pathway |
| NCBI Summary: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014] |
| UniProt Code: | P15336 |
| NCBI GenInfo Identifier: | 215274241 |
| NCBI Gene ID: | 1386 |
| NCBI Accession: | P15336.4 |
| UniProt Secondary Accession: | P15336,Q13000, Q3B7B7, Q4ZFU9, Q53RY2, Q8TAR1, A1L3Z2 A4D7U4, A4D7U5, A4D7V1, D3DPE9, G8JLM5, |
| UniProt Related Accession: | P15336 |
| Molecular Weight: | 505 |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-2 |
| NCBI Synonym Full Names: | activating transcription factor 2 |
| NCBI Official Symbol: | ATF2 |
| NCBI Official Synonym Symbols: | HB16; CREB2; TREB7; CREB-2; CRE-BP1 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-2; histone acetyltransferase ATF2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive element binding protein 2, formerly; activating transcription factor 2 splice variant ATF2-var2 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-2 |
| UniProt Synonym Protein Names: | Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1 |
| Protein Family: | Alcohol O-acetyltransferase |
| UniProt Gene Name: | ATF2 |
| UniProt Entry Name: | ATF2_HUMAN |
