Description
ATF3 Transcription Factor Activity Assay
The ATF3 Transcription Factor Activity Assay is a powerful tool for studying the transcriptional activity of ATF3, a key transcription factor involved in cellular stress response and regulation of gene expression. This assay allows researchers to accurately measure ATF3 activity in cell lysates, providing valuable insights into cellular signaling pathways and transcriptional regulation.With its high sensitivity and specificity, the ATF3 Transcription Factor Activity Assay delivers reliable and reproducible results, making it ideal for a wide range of research applications.
By understanding the role of ATF3 in various physiological and pathological processes, researchers can uncover new insights into diseases such as cancer, inflammation, and metabolic disorders.Overall, the ATF3 Transcription Factor Activity Assay is a valuable tool for researchers looking to unravel the complex mechanisms of gene regulation and cellular stress response, ultimately advancing our understanding of disease pathogenesis and paving the way for potential therapeutic interventions.
| Product Name: | ATF3 Transcription Factor Activity Assay |
| Product Code: | TFAB00084 |
| Target: | ATF3 |
| Synonyms: | Activating transcription factor 3 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ATF3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 1q32.3 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011] |
| UniProt Code: | P18847 |
| NCBI GenInfo Identifier: | 1168543 |
| NCBI Gene ID: | 467 |
| NCBI Accession: | P18847.2 |
| UniProt Secondary Accession: | P18847,Q5VTZ2, Q6ICQ9, Q7Z566, Q8WYM6, |
| UniProt Related Accession: | P18847 |
| Molecular Weight: | 14,314 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| NCBI Synonym Full Names: | activating transcription factor 3 |
| NCBI Official Symbol: | ATF3 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Synonym Protein Names: | Activating transcription factor 3 |
| Protein Family: | Activating transcription factor |
| UniProt Gene Name: | ATF3 |
| UniProt Entry Name: | ATF3_HUMAN |
