The ATG4D Antibody (CAB7842) is a high-quality antibody developed for reliable detection and analysis of target proteins. Autophagy is a cellular process that degrades and recycles damaged organelles and proteins, playing a critical role in maintaining cellular health and function. The antibody, produced in rabbits, demonstrates high specificity and sensitivity when detecting ATG4D in human samples, making it a reliable tool for Western blot applications.ATG4D, a member of the autophagy-related 4 (ATG4) family, is essential for the processing of LC3 proteins during autophagosome formation. Dysregulation of ATG4D has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
ATG4D Antibody
SKU:
CAB7842
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG4D, AUTL4, APG4-D, HsAPG4D, ATG4D
Positive Sample:
Rat skeletal muscle
Cellular Localization:
Cytoplasm, Mitochondrion Matrix.
Calculated MW:
53kDa
Observed MW:
53kDa
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
84971
RRID
AB_2768492
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat skeletal muscle using ATG4D Rabbit pAb (CAB7842) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
