Anti-ATG5 Antibody (CAB0203)
- SKU:
- CAB0203
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-ATG5 Antibody |
| Antibody SKU: | CAB0203 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTDD KDVL RDVW FGRI PTCF TLYQ DEIT EREA EPYY LLLP RVSY LTLV TDKV KKHF QKVM RQED ISEI WFEY EGTP LKWH YPIG LLFD LLAS SSAL PWNI TVHF KSFP EKDL LHCP SKDA IEAH FMSC MKEA DALK HKSQ VINE MQKK DHKQ LWMG LQND RFDQ FWAI NRKL MEYP AEEN GFRY IPFR IYQT TTER PFIQ KLFR PVAA DGQL HTLG DLLK EVCP SAID PEDG EKKN QVMI HGIE PMLE TPLQ WLSE HLSY PDNF LHIS IIPQ PTD |
| Gene ID: | 9474 |
| Uniprot: | Q9H1Y0 |
| Cellular Location: | Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane |
| Calculated MW: | 22kDa/32kDa |
| Observed MW: | 55kDa |
| Synonyms: | ATG5, APG5, APG5-LIKE, APG5L, ASP, hAPG5 |
| Background: | The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. |
| UniProt Code: | Q9H1Y0 |
| NCBI GenInfo Identifier: | 17366828 |
| NCBI Gene ID: | 9474 |
| NCBI Accession: | Q9H1Y0.2 |
| UniProt Secondary Accession: | Q9H1Y0,O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7, |
| UniProt Related Accession: | Q9H1Y0 |
| Molecular Weight: | 275 |
| NCBI Full Name: | Autophagy protein 5 |
| NCBI Synonym Full Names: | autophagy related 5 |
| NCBI Official Symbol: | ATG5Â Â |
| NCBI Official Synonym Symbols: | ASP; APG5; APG5L; hAPG5; APG5-LIKEÂ Â |
| NCBI Protein Information: | autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog |
| UniProt Protein Name: | Autophagy protein 5 |
| UniProt Synonym Protein Names: | APG5-like; Apoptosis-specific protein |
| Protein Family: | Autophagy protein |
| UniProt Gene Name: | ATG5Â Â |
| UniProt Entry Name: | ATG5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG5 antibody (CAB0203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat kidney using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using ATG5 antibody (CAB0203) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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