Anti-ATP6V1G3 Antibody (CAB14443)

Antibody Name:	Anti-ATP6V1G3 Antibody
Antibody SKU:	CAB14443
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human ATP6V1G3 (NP_573569.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human ATP6V1G3 (NP_573569.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSQ SQGI HQLL QAEK RAKD KLEE AKKR KGKR LKQA KEEA MVEI DQYR MQRD KEFR LKQS KIMG SQNN LSDE IEEQ TLGK IQEL NGHY NKYM ESVM NQLL SMVC DMKP EIHV NYRA TN
Gene ID:	127124
Uniprot:	Q96LB4
Cellular Location:
Calculated MW:	6kDa/13kDa/14kDa
Observed MW:	14kDa

Synonyms:

ATP6V1G3, ATP6G3, Vma10

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Function: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
UniProt Protein Details:	Subunit structure: V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d) By similarity. Tissue specificity: Kidney. Ref.1 Sequence similarities: Belongs to the V-ATPase G subunit family.
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96LB4
NCBI GenInfo Identifier:	72533514
NCBI Gene ID:	127124
NCBI Accession:	AAI01130.1
UniProt Related Accession:	Q96LB4
Molecular Weight:
NCBI Full Name:	ATP6V1G3 protein
NCBI Synonym Full Names:	ATPase H+ transporting V1 subunit G3
NCBI Official Symbol:	ATP6V1G3
NCBI Official Synonym Symbols:	Vma10; ATP6G3
NCBI Protein Information:	V-type proton ATPase subunit G 3
UniProt Protein Name:	V-type proton ATPase subunit G 3
UniProt Synonym Protein Names:	V-ATPase 13 kDa subunit 3; Vacuolar proton pump subunit G 3
UniProt Gene Name:	ATP6V1G3
UniProt Entry Name:	VATG3_HUMAN

	Western blot analysis of extracts of mouse kidney, using ATP6V1G3 antibody (CAB14443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using ATP6V1G3 antibody (CAB14443) at dilution of 1:100 (40x lens).

Antibodies

ATP6V1G3 Antibody (PACO07943)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)