Anti-ASNA1 Antibody (CAB3746)

Antibody Name:	Anti-ASNA1 Antibody
Antibody SKU:	CAB3746
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ASNA1 (NP_004308.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, MCF7, 293T, Mouse kidney, Mouse testis, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ASNA1 (NP_004308.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAG VAGW GVEA EEFE DAPD VEPL EPTL SNII EQRS LKWI FVGG KGGV GKTT CSCS LAVQ LSKG RESV LIIS TDPA HNIS DAFD QKFS KVPT KVKG YDNL FAME IDPS LGVA ELPD EFFE EDNM LSMG KKMM QEAM SAFP GIDE AMSY AEVM RLVK GMNF SVVV FDTA PTGH TLRL LNFP TIVE RGLG RLMQ IKNQ ISPF ISQM CNML GLGD MNAD QLAS KLEE TLPV IRSV SEQF KDPE QTTF ICVC IAEF LSLY ETER LIQE LAKC KIDT HNII VNQL VFPD PEKP CKMC EARH KIQA KYLD QMED LYED FHIV KLPL LPHE VRGA DKVN TFSA LLLE PYKP PSAQ
Gene ID:	439
Uniprot:	O43681
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Nucleus, nucleolus
Calculated MW:	38kDa
Observed MW:	39kDa

Synonyms:

ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, TRC40, hASNA-I

Background:

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion.

UniProt Protein Function:	ASNA1: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. Belongs to the arsA ATPase family.
UniProt Protein Details:	Protein type:Nucleolus; EC 3.6.-.-; EC 3.6.3.16; Hydrolase Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: endoplasmic reticulum; cytoplasm; nucleolus; nucleus Molecular Function:arsenite transmembrane transporter activity; transporter activity; metal ion binding; ATPase activity; ATP binding Biological Process: protein insertion into ER membrane; transport; metabolic process; inorganic anion transport
NCBI Summary:	This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]
UniProt Code:	O43681
NCBI GenInfo Identifier:	6647417
NCBI Gene ID:	439
NCBI Accession:	O43681.2
UniProt Secondary Accession:	O43681,Q53FC6, Q92849, A6NHP8, A8K740,
UniProt Related Accession:	O43681
Molecular Weight:	Calculated: 38kDaObserved: 39kDa
NCBI Full Name:	ATPase ASNA1
NCBI Synonym Full Names:	arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
NCBI Official Symbol:	ASNA1
NCBI Official Synonym Symbols:	GET3; ARSA1; TRC40; ARSA-I; ASNA-I; hASNA-I
NCBI Protein Information:	ATPase ASNA1; golgi to ER traffic 3 homolog; transmembrane domain recognition complex, 40kDa; transmembrane domain recognition complex 40 kDa ATPase subunit
UniProt Protein Name:	ATPase ASNA1
UniProt Synonym Protein Names:	Arsenical pump-driving ATPase; Arsenite-stimulated ATPase
Protein Family:	ATPase
UniProt Gene Name:	ASNA1
UniProt Entry Name:	ASNA_HUMAN

	Western blot analysis of extracts of various cell lines, using ASNA1 antibody (CAB3746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using ASNA1 antibody (CAB3746) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ASNA1 Rabbit pAb (CAB3746) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using ASNA1 Rabbit pAb (CAB3746) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ASNA1 Antibody (PACO07886)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)