Anti-BACH1 Antibody (CAB5393)

Antibody Name:	Anti-BACH1 Antibody
Antibody SKU:	CAB5393
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human BACH1 (NP_996749.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human BACH1 (NP_996749.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLS ENSV FAYE SSVH STNV LLSL NDQR KKDV LCDV TIFV EGQR FRAH RSVL AACS SYFH SRIV GQAD GELN ITLP EEVT VKGF EPLI QFAY TAKL ILSK ENVD EVCK CVEF LSVH NIEE SCFQ FLKF KFLD STAD QQEC PRKK CFSS HCQK TDLK LSLL DQRD LETD EVEE FLEN KNVQ TPQC KLRR YQGN AKAS PPLQ DSAS QTYE SMCL EKDA ALAL PSLC PKYR KFQK AFGT DRVR TGES SVKD IHAS VQPN ERSE NECL GGVP ECRD LQVM LKCD ESKL AMEP EETK KDPA SQCP TEKS EVTP FPHN SSID PHGL
Gene ID:	571
Uniprot:	O14867
Cellular Location:	Nucleus
Calculated MW:	81kDa
Observed MW:	115kDa

Synonyms:

BACH1, BACH-1, BTBD24

Background:

This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.

UniProt Protein Function:	BACH1: a transcriptional regulator that acts as a repressor or activator. Binds, in-vitro, to NF-E2 binding sites on DNA. Plays an important role in coordinating transcription activation and repression by NF-E2. Belongs to the bZIP family, CNC subfamily.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 21q22.11 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; transcription factor activity; ubiquitin-protein ligase activity Biological Process: DNA repair; G1/S-specific transcription in mitotic cell cycle; G2/M-specific transcription in mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009]
UniProt Code:	O14867
NCBI GenInfo Identifier:	6686266
NCBI Gene ID:	571
NCBI Accession:	O14867.2
UniProt Secondary Accession:	O14867,O43285, Q6ICU0,
UniProt Related Accession:	O14867
Molecular Weight:	81,958 Da
NCBI Full Name:	Transcription regulator protein BACH1
NCBI Synonym Full Names:	BTB domain and CNC homolog 1
NCBI Official Symbol:	BACH1
NCBI Official Synonym Symbols:	BACH-1; BTBD24
NCBI Protein Information:	transcription regulator protein BACH1
UniProt Protein Name:	Transcription regulator protein BACH1
UniProt Synonym Protein Names:	BTB and CNC homolog 1; HA2303
Protein Family:	Transcription regulator protein
UniProt Gene Name:	BACH1
UniProt Entry Name:	BACH1_HUMAN

	Western blot analysis of extracts of various cell lines, using BACH1 antibody (CAB5393) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using BACH1 Rabbit pAb (CAB5393) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using BACH1 Rabbit pAb (CAB5393) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using BACH1 Rabbit pAb (CAB5393) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using BACH1 antibody (CAB5393) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
BACH1 Antibody (PACO00451)	BACH1 Colorimetric Cell-Based ELISA
BACH1 Antibody (PACO07972)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)