The Bovine Histone H3 ELISA Kit (BOFI00112) is a reliable and accurate tool for the detection of bovine histone H3 levels in various biological samples. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in research applications. Histone H3 is a key protein involved in chromatin structure and gene regulation, playing a critical role in various cellular processes such as transcription, DNA repair, and cell division.
Abnormal levels of histone H3 have been linked to various diseases and abnormalities, making it an important biomarker for studying disease mechanisms and potential therapeutic interventions. With its user-friendly protocol and high-performance characteristics, the Bovine Histone H3 ELISA Kit (BOFI00112) is an invaluable tool for researchers seeking to investigate the role of histone H3 in biological processes and disease development.
Product Name:
Bovine Histon H3 ELISA Kit
SKU:
BOFI00112
Reactivity:
Bovine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-101%
86-99%
92-101%
EDTA Plasma (n = 5)
86-98%
85-101%
83-99%
Heparin Plasma (n = 5)
84-97%
85-100%
82-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-103
95
EDTA Plasma (n = 5)
85-102
96
Heparin Plasma (n = 5)
85-101
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
