The Bovine Ubiquitin ELISA Kit from Assay Genie is a comprehensive and reliable tool for the quantification of ubiquitin levels in bovine serum, plasma, and cell lysates. With its high specificity and sensitivity, this kit provides accurate and consistent results, making it an essential asset for researchers studying ubiquitin-mediated processes.Ubiquitin is a highly conserved protein that plays a pivotal role in protein degradation, immune response, and DNA repair mechanisms. Dysregulation of ubiquitin pathways has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
This ELISA kit allows for the precise measurement of ubiquitin levels, facilitating research into the underlying mechanisms of these diseases and the development of targeted therapies.Whether investigating protein homeostasis, cellular signaling, or disease pathogenesis, the Bovine Ubiquitin ELISA Kit offers a valuable tool for researchers seeking to deepen their understanding of ubiquitin biology. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Bovine Ubiquitin (Ub) ELISA Kit
Product Code:
BODL00182
Size:
96 Assays
Target:
Ub
Synonyms:
Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-100
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
