The Chicken Ubiquitin (UB) ELISA Kit is a reliable and accurate tool for measuring levels of ubiquitin in chicken samples. Ubiquitin is a small protein involved in protein degradation and regulation, playing a key role in various cellular processes.With high sensitivity and specificity, this ELISA kit delivers precise and reproducible results, making it suitable for a wide range of research applications in the field of cellular biology and molecular biology. Understanding ubiquitin levels in chicken samples can provide valuable insights into protein regulation mechanisms and potential implications for various diseases and disorders.
This kit can be used in studies related to cell signaling, protein degradation pathways, and cellular stress responses.Overall, the Chicken Ubiquitin (UB) ELISA Kit is a valuable tool for researchers seeking to explore the role of ubiquitin in chicken biology and its potential relevance in various physiological and pathological processes.
Product Name:
Chicken Ubiquitin (Ub) ELISA Kit
Product Code:
CHDL00053
Size:
96 Assays
Target:
Ub
Synonyms:
Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
