Anti-BRMS1 Antibody (CAB14865)

Antibody Name:	Anti-BRMS1 Antibody
Antibody SKU:	CAB14865
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEEV GAER APEY TEPL GGLQ RSLK IRIQ VAGI YKGF CLDV IRNK YECE LQGA KQHL ESEK LLLY DTLQ GELQ ERIQ RLEE DRQS LDLS SEWW DDKL HARG SSRS WDSL PPSK RKKA PLVS G
Gene ID:	25855
Uniprot:	Q9HCU9
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

BRMS1

Background:

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

UniProt Protein Function:	BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family.
UniProt Protein Details:	Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; protein binding Biological Process: histone deacetylation; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9HCU9
NCBI GenInfo Identifier:	18202959
NCBI Gene ID:	25855
NCBI Accession:	Q9HCU9.1
UniProt Secondary Accession:	Q9HCU9,Q6IAI2,
UniProt Related Accession:	Q9HCU9
Molecular Weight:	28,461 Da
NCBI Full Name:	Breast cancer metastasis-suppressor 1
NCBI Synonym Full Names:	breast cancer metastasis suppressor 1
NCBI Official Symbol:	BRMS1
NCBI Protein Information:	breast cancer metastasis-suppressor 1
UniProt Protein Name:	Breast cancer metastasis-suppressor 1
Protein Family:	Breast cancer metastasis-suppressor
UniProt Gene Name:	BRMS1
UniProt Entry Name:	BRMS1_HUMAN

	Western blot analysis of extracts of various cell lines, using BRMS1 antibody (CAB14865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of H9C2 cells using BRMS1 Rabbit pAb (CAB14865) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

BRMS1 Antibody (PACO00476)

BRMS1 Antibody (PACO01872)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)