Anti-BYSL Antibody (CAB11993)

Antibody Name:	Anti-BYSL Antibody
Antibody SKU:	CAB11993
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_004044.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, A-549, BxPC-3, Mouse testis, Mouse lung, Rat testis, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_004044.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HAEV VVDP EDER AIEM FMNK NPPA RRTL ADII MEKL TEKQ TEVE TVMS EVSG FPMP QLDP RVLE VYRG VREV LSKY RSGK LPKA FKII PALS NWEQ ILYV TEPE AWTA AAMY QATR IFAS NLKE RMAQ RFYN LVLL PRVR DDVA EYKR LNFH LYMA LKKA L
Gene ID:	705
Uniprot:	Q13895
Cellular Location:
Calculated MW:	49kDa
Observed MW:	49kDa

Synonyms:

BYSL, BYSTIN, bystin

Background:

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.

NCBI Summary:	Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13895
NCBI GenInfo Identifier:	51173724
NCBI Gene ID:	705
NCBI Accession:	NP_004044.3
UniProt Related Accession:	Q13895
Molecular Weight:	49kDa
NCBI Full Name:	bystin
NCBI Synonym Full Names:	bystin like
NCBI Official Symbol:	BYSL
NCBI Official Synonym Symbols:	Enp1; BYSTIN
NCBI Protein Information:	bystin
UniProt Protein Name:	Bystin
Protein Family:	Bystin
UniProt Gene Name:	BYSL
UniProt Entry Name:	BYST_HUMAN

	Western blot analysis of extracts of various cell lines, using BYSL antibody (CAB11993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat ovary using BYSL antibody (CAB11993) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using BYSL antibody (CAB11993) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using BYSL antibody (CAB11993) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)