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Anti-CAPN5 Antibody (CAB7428)

SKU:
CAB7428
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 11 Anti-CAPN5 Antibody CAB7428
  • Cell Biology Antibodies 11 Anti-CAPN5 Antibody CAB7428
€139 - €419
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Description

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Antibody Name:Anti-CAPN5 Antibody
Antibody SKU:CAB7428
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, Mouse thymus, Mouse brain, Mouse spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GAWT LHED PRQN RGGG CINH KDTF FQNP QYIF EVKK PEDE VLIC IQQR PKRS TRRE GKGE NLAI GFDI YKVE ENRQ YRMH SLQH KAAS SIYI NSRS VFLR TDQP EGRY VIIP TTFE PGHT GEFL LRVF TDVP SNCR ELRL DEPP HTCW SSLC GYPQ LVTQ VHVL GAAG LKDS PTGA NSYV IIKC EGDK VRSA VQKG TSTP EYNV KGIF YRKK LSQP ITVQ VWNH RVLK DEFL GQVH LKAD PDNL QALH TLHL RDRN SRQP SNLP GTVA VHIL SSTS LMAV
Gene ID:726
Uniprot:O15484
Cellular Location:
Calculated MW:73kDa
Observed MW:73kDa
Synonyms:CAPN5, ADNIV, HTRA3, VRNI, nCL-3, calpain-5
Background:Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.
UniProt Protein Function:CAPN5: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: cell surface; focal adhesion; cytoplasm

Molecular Function:calcium-dependent cysteine-type endopeptidase activity

Biological Process: proteolysis; signal transduction

Disease: Vitreoretinopathy, Neovascular Inflammatory

NCBI Summary:Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
UniProt Code:O15484
NCBI GenInfo Identifier:28376972
NCBI Gene ID:726
NCBI Accession:O15484.2
UniProt Secondary Accession:O15484,O00263,
UniProt Related Accession:O15484
Molecular Weight:640
NCBI Full Name:Calpain-5
NCBI Synonym Full Names:calpain 5
NCBI Official Symbol:CAPN5  
NCBI Official Synonym Symbols:VRNI; ADNIV; HTRA3; nCL-3  
NCBI Protein Information:calpain-5; new calpain 3; calpain htra-3
UniProt Protein Name:Calpain-5
UniProt Synonym Protein Names:Calpain htra-3; New calpain 3; nCL-3
Protein Family:Calpain
UniProt Gene Name:CAPN5  
UniProt Entry Name:CAN5_HUMAN
Anti-CAPN5 Antibody (CAB7428)Western blot analysis of extracts of various cell lines, using CAPN5 antibody (CAB7428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-CAPN5 Antibody (CAB7428)Immunofluorescence analysis of NIH-3T3 cells using CAPN5 antibody (CAB7428) at dilution of 1:100. Blue: DAPI for nuclear staining.

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