Description
Canine Chemokine Array (9 targets) (SARB0007)
The Canine Chemokine Array (SARb0007) is a powerful research tool for studying canine chemokines involved in inflammation and immune response in dogs. This array allows for the simultaneous detection of 9 different targets, providing comprehensive insight into the chemokine profile of canine samples.With high sensitivity and specificity, this array is ideal for various research applications including biomarker discovery, drug development, and disease pathogenesis studies in veterinary medicine. By identifying and quantifying key chemokines, researchers can better understand the immune response in dogs, leading to the development of targeted therapies for conditions such as infectious diseases, cancer, and autoimmune disorders.
The Canine Chemokine Array offers a streamlined and efficient way to analyze multiple targets in a single experiment, saving time and resources while providing valuable data for advancing canine health research. Take advantage of this cutting-edge technology to explore the intricate interplay of chemokines in canine immunity and disease, driving progress towards improved diagnosis and treatment strategies for our beloved canine companions.
| Product Sku: | SARB0007 |
| Size: | 2, 4, or 8 |
| Species Detected: | Canine |
| Number of Targets Detected: | 9 |
| Gene Symbols: | CCL2, CCL4, CXCL1, CXCL8, IFNG, IL1B, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GRO alpha (CXCL1)
| IFN-gamma
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2)
| IL-8 (CXCL8)
|
MCP-1 (CCL2)
| MIP-1 beta (CCL4)
| RANTES (CCL5) | TNF alpha
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
