Anti-CANX Antibody (CAB15631)

Antibody Name:	Anti-CANX Antibody
Antibody SKU:	CAB15631
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 501-592 of human CANX (NP_001737.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 501-592 of human CANX (NP_001737.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FCCS GKKQ TSGM EYKK TDAP QPDV KEEE EEKE EEKD KGDE EEEG EEKL EEKQ KSDA EEDG GTVS QEEE DRKP KAEE DEIL NRSP RNRK PRRE
Gene ID:	821
Uniprot:	P27824
Cellular Location:	Endoplasmic reticulum, Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein
Calculated MW:	55kDa/67kDa/71kDa
Observed MW:	90KDa

Synonyms:

CNX, IP90, P90, Calnexin, CANX, calnexin

Background:

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

UniProt Protein Function:	Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum.Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Calcium-binding; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q35 Cellular Component: dendrite cytoplasm; endoplasmic reticulum membrane; protein complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; dendritic spine; cell soma; membrane; axon; melanosome; ribosome Molecular Function:ionotropic glutamate receptor binding; protein binding; unfolded protein binding; apolipoprotein binding; calcium ion binding; carbohydrate binding; glycoprotein binding Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; synaptic vesicle endocytosis; protein secretion; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging
NCBI Summary:	This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P27824
NCBI GenInfo Identifier:	543920
NCBI Gene ID:	821
NCBI Accession:	P27824.2
UniProt Secondary Accession:	P27824,B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3,
UniProt Related Accession:	P27824
Molecular Weight:	55,598 Da
NCBI Full Name:	Calnexin
NCBI Synonym Full Names:	calnexin
NCBI Official Symbol:	CANX
NCBI Official Synonym Symbols:	CNX; P90; IP90
NCBI Protein Information:	calnexin; major histocompatibility complex class I antigen-binding protein p88
UniProt Protein Name:	Calnexin
UniProt Synonym Protein Names:	IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
UniProt Gene Name:	CANX
UniProt Entry Name:	CALX_HUMAN

	Western blot analysis of extracts of SW480 cells, using CANX Rabbit pAb (CAB15631) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using CANX antibody (CAB15631) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using CANX antibody (CAB15631) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug CANX antibody (CAB15631). Western blot was performed from the immunoprecipitate using CANX antibody (CAB15631) at a dilition of 1:500.

Antibodies	ELISA Kits
Anti-Calnexin Antibody (CAB4846)	Human CNX (Calnexin) ELISA Kit (HUES01789)
Phospho-CANX (S583) Antibody (PACO00192)	Human Calnexin (CANX) ELISA Kit
CANX Antibody (PACO06312)	Calnexin Colorimetric Cell-Based ELISA Kit
CANX Antibody (PACO07037)	Calnexin (Phospho-Ser583) Colorimetric Cell-Based ELISA Kit
CANX Antibody (PACO08164)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)