Anti-CAPZB Antibody (CAB8106)

Antibody Name:	CAPZB Rabbit Polyclonal Antibody
Antibody SKU:	CAB8106
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human CAPZB (NP_004921.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, THP-1, HeLa, Mouse brain, Mouse testis, Mouse spleen, Rat small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human CAPZB (NP_004921.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSDQ QLDC ALDL MRRL PPQQ IEKN LSDL IDLV PSLC EDLL SSVD QPLK IARD KVVG KDYL LCDY NRDG DSYR SPWS NKYD PPLE DGAM PSAR LRKL EVEA NNAF DQYR DLYF EGGV SSVY LWDL DHGF AGVI LIKK AGDG SKKI KGCW DSIH VVEV QEKS SGRT AHYK LTST VMLW LQTN KSGS GTMN LGGS LTRQ MEKD ETVS DCSP HIAN IGRL VEDM ENKI RSTL NEIY FGKT KDIV NGLR SVQT FADK SKQE ALKN DLVE ALKR KQQC
Gene ID:	832
Uniprot:	P47756
Cellular Location:	Cytoplasm, cytoskeleton, myofibril, sarcomere
Calculated MW:	30kDa/31kDa
Observed MW:	31kDa

Synonyms:

CAPZB, CAPB, CAPPB, CAPZ

Background:

This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

UniProt Protein Function:	CAPZB: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization. Belongs to the F-actin-capping protein beta subunit family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: actin cytoskeleton; actin filament; cytoskeleton; cytosol Molecular Function:actin binding; actin filament binding Biological Process: barbed-end actin filament capping; blood coagulation; cell motility; cytoskeleton organization and biogenesis; negative regulation of filopodium formation; regulation of cell morphogenesis
NCBI Summary:	This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, Aug 2013]
UniProt Code:	P47756
NCBI GenInfo Identifier:	13124696
NCBI Gene ID:	832
NCBI Accession:	P47756.4
UniProt Secondary Accession:	P47756,Q32Q68, Q5U0L4, Q8TB49, Q9NUC4,
UniProt Related Accession:	P47756
Molecular Weight:	Da
NCBI Full Name:	F-actin-capping protein subunit beta
NCBI Synonym Full Names:	capping actin protein of muscle Z-line beta subunit
NCBI Official Symbol:	CAPZB
NCBI Official Synonym Symbols:	CAPB; CAPZ; CAPPB
NCBI Protein Information:	F-actin-capping protein subunit beta
UniProt Protein Name:	F-actin-capping protein subunit beta
UniProt Synonym Protein Names:	CapZ beta
Protein Family:	F-actin-capping protein
UniProt Gene Name:	CAPZB
UniProt Entry Name:	CAPZB_HUMAN

	Western blot analysis of extracts of various cell lines, using CAPZB antibody (CAB8106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using CAPZB antibody (CAB8106) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)