Anti-CARD10 Antibody (CAB7368)

Antibody Name:	Anti-CARD10 Antibody
Antibody SKU:	CAB7368
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, HT-29, Jurkat, 293T, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLDL PSSR LDFQ VCPA ESLS GEEL CPSS APGA PKAQ PATP GLGS RIRA IQES VGKK HCLL ELGA RGVR ELVQ NEIY PIVI HVEV TEKN VREV RGLL GRPG WRDS ELLR QCRG SEQV LWGL PCSW VQVP AHEW GHAE ELAK VVRG RILQ EQAR LVWV ECGS SRGC PSSS EA
Gene ID:	29775
Uniprot:	Q9BWT7
Cellular Location:	Cytoplasm
Calculated MW:	82kDa/115kDa
Observed MW:	80kDa

Synonyms:	CARD10, BIMP1, CARMA3
Background:

UniProt Protein Function:	CARD10: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 22q13.1 Molecular Function:protein binding Biological Process: activation of NF-kappaB-inducing kinase
NCBI Summary:	The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9BWT7
NCBI GenInfo Identifier:	116241281
NCBI Gene ID:	29775
NCBI Accession:	Q9BWT7.2
UniProt Secondary Accession:	Q9BWT7,Q14CQ8, Q5TFG6, Q8NC81, Q9UGR5, Q9UGR6, Q9Y3H0
UniProt Related Accession:	Q9BWT7
Molecular Weight:	82,541 Da
NCBI Full Name:	Caspase recruitment domain-containing protein 10
NCBI Synonym Full Names:	caspase recruitment domain family member 10
NCBI Official Symbol:	CARD10
NCBI Official Synonym Symbols:	BIMP1; CARMA3
NCBI Protein Information:	caspase recruitment domain-containing protein 10
UniProt Protein Name:	Caspase recruitment domain-containing protein 10
UniProt Synonym Protein Names:	CARD-containing MAGUK protein 3; Carma 3
Protein Family:	Caspase recruitment domain-containing protein
UniProt Gene Name:	CARD10
UniProt Entry Name:	CAR10_HUMAN

	Western blot analysis of extracts of various cell lines, using CARD10 antibody (CAB7368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using CARD10 antibody (CAB7368) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CARD10 antibody (CAB7368) at dilution of 1:100 (40x lens).

Antibodies

CARD10 Antibody (PACO00509)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)