Anti-CBR1 Antibody (CAB5446)
- SKU:
- CAB5446
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CBR1 Antibody |
| Antibody SKU: | CAB5446 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CBR1 (NP_001748.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SW480, 22Rv1, MCF7, NCI-H460, SKOV3, Mouse kidney, Mouse liver, Mouse lung, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CBR1 (NP_001748.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSG IHVA LVTG GNKG IGLA IVRD LCRL FSGD VVLT ARDV TRGQ AAVQ QLQA EGLS PRFH QLDI DDLQ SIRA LRDF LRKE YGGL DVLV NNAG IAFK VADP TPFH IQAE VTMK TNFF GTRD VCTE LLPL IKPQ GRVV NVSS IMSV RALK SCSP ELQQ KFRS ETIT EEEL VGLM NKFV EDTK KGVH QKEG WPSS AYGV TKIG VTVL SRIH ARKL SEQR KGDK ILLN ACCP GWVR TDMA GPKA TKSP EEGA ETPV YLAL LPPD AEGP HGQF VSEK RVEQ W |
| Gene ID: | 873 |
| Uniprot: | P16152 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 18kDa/30kDa |
| Observed MW: | 35kDa |
| Synonyms: | CBR1, CBR, SDR21C1, hCBR1 |
| Background: | The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| UniProt Protein Details: | Protein type:EC 1.1.1.189; Lipid Metabolism - arachidonic acid; EC 1.1.1.197; Oxidoreductase; EC 1.1.1.184 Chromosomal Location of Human Ortholog: 21q22.13 Cellular Component: cytosol; vesicle Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity; 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity Biological Process: epithelial cell differentiation; vitamin K metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; drug metabolic process |
| NCBI Summary: | The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013] |
| UniProt Code: | P16152 |
| NCBI GenInfo Identifier: | 118519 |
| NCBI Gene ID: | 873 |
| NCBI Accession: | P16152.3 |
| UniProt Secondary Accession: | P16152,Q3LHW8, B2RBZ7, B4DFK7, |
| UniProt Related Accession: | P16152 |
| Molecular Weight: | 277 |
| NCBI Full Name: | Carbonyl reductase |
| NCBI Synonym Full Names: | carbonyl reductase 1 |
| NCBI Official Symbol: | CBR1Â Â |
| NCBI Official Synonym Symbols: | CBR; hCBR1; SDR21C1Â Â |
| NCBI Protein Information: | carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1 |
| UniProt Protein Name: | Carbonyl reductase [NADPH] 1 |
| UniProt Synonym Protein Names: | 15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197); NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase (EC:1.1.1.189) |
| Protein Family: | Carbonyl reductase |
| UniProt Gene Name: | CBR1Â Â |
| UniProt Entry Name: | CBR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CBR1 antibody (CAB5446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of A-549 cells using CBR1 antibody (CAB5446). Blue: DAPI for nuclear staining. |
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