The CD248 Antibody (CAB12706) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CD248 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it well-suited for studies in immunology and cancer research.CD248, also known as endosialin, is an important regulator of vascular development and fibrosis, with implications in tumor growth and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD248 Antibody
SKU:
CAB12706
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TEM1, CD164L1, CD248
Positive Sample:
HeLa
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
81kDa
Observed MW:
81kDa
Predicted to enable extracellular matrix binding activity and extracellular matrix protein binding activity. Predicted to be involved in cell migration. Predicted to act upstream of or within several processes, including anatomical structure regression; lymph node development; and positive regulation of endothelial cell apoptotic process. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
57124
RRID
AB_2759548
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using CD248 Rabbit pAb (CAB12706) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
