Anti-CD4 (no azide) Antibody (AGIM0124)

CD4 (no azide) monoclonal antibody (AGIM0124)

SKU:

AGIM0124

Product Type:

Antibody

Antibody Type:

Monoclonal Antibody

Reactivity:

Human

Host Species:

Mouse

Isotype:

IgG2a

Clone:

EDU-2

Conjugation:

Unconjugated

Applications:

Description

Description

Datasheet

Isotype:	IgG2a
Host Species:	Mouse
Storage:	Aliquot and store at -20°C. Avoid freeze/thaw cycles. Should be handled underaseptic conditions.
Uniprot:	P01730
Background:	CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-Like V-type and 3 Ig-like C2-Type). Transmembrane region forms 25aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described, that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1; HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin; Intracellular ligands: p56Lck; CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; Cd4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, Tcell selection and signal transduction. Defects in antigen presentation (MHC classII) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodefiency). This material is offered for research only. Not for use in human. For in vitro use only. ImmunoTools will not be held responsible for patent infringement or other violations that may occur with the use of our products. .

UniProt Protein Function:	CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 12p13.31 Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft Molecular Function:protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation Disease: Okt4 Epitope Deficiency
NCBI Summary:	This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
UniProt Code:	P01730
NCBI GenInfo Identifier:	116013
NCBI Gene ID:	920
NCBI Accession:	P01730.1
UniProt Secondary Accession:	P01730,Q4ZGK2, Q5U066, Q9UDE5, B2R737, D3DUS5,
UniProt Related Accession:	P01730
Molecular Weight:	458
NCBI Full Name:	T-cell surface glycoprotein CD4
NCBI Synonym Full Names:	CD4 molecule
NCBI Official Symbol:	CD4
NCBI Official Synonym Symbols:	CD4mut
NCBI Protein Information:	T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
UniProt Protein Name:	T-cell surface glycoprotein CD4
UniProt Synonym Protein Names:	T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family:	CD40 ligand
UniProt Gene Name:	CD4
UniProt Entry Name:	CD4_HUMAN